Low copy number gene transfer and stable expression in a commercial wheat cultivar via particle bombardment

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Low copy number gene transfer and stable expression in a commercial wheat cultivar via particle bombardment.

Two groups of linear gene constructs (gus and bar, and 1Ax1 and bar) lacking vector backbone sequences were independently transferred into the elite wheat (Triticum aestivum L.) variety EM12, and genetically stable transgenic plants with low copy number transgene integration were recovered. Co-transformation experiments were carried out in parallel using either circular whole plasmid(s) or line...

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Copy number variants, diseases and gene expression.

Copy number variation (CNV) has recently gained considerable interest as a source of genetic variation likely to play a role in phenotypic diversity and evolution. Much effort has been put into the identification and mapping of regions that vary in copy number among seemingly normal individuals in humans and a number of model organisms, using bioinformatics or hybridization-based methods. These...

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ژورنال

عنوان ژورنال: Journal of Experimental Botany

سال: 2006

ISSN: 0022-0957,1460-2431

DOI: 10.1093/jxb/erl145